Picture of StepOnePlus real-time PCR
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"Bertha" is our StepOnePlus machine for real-time/quantitative PCR. 

The StepOnePlus can do both real-time runs or end-point runs.

Bertha is connected to a laptop with the StepOne software. It is also possible to run Bertha as a stand-alonge system.

 

The system has four filters that can detect these fluorophores:

Filter Dye emission
1

FAM

SYBR Green

~ 520 nm
2

JOE

VIC

~ 550 nm
3

TAMRA

NED

~ 580 nm
4 ROX ~ 610 nm

 

Extensive manuals for the following types of experiments are found in the biology lab's index file:

  • Standard curve experiments
  • Relative standard curve and comparative CT experiments
  • Genotyping experiments
  • Precence/absence experiments
Tool name:
StepOnePlus real-time PCR
Area/room:
G1-21 Biology
Category:
Chemical and Biological methods
Manufacturer:
Applied Biosystems
Model:
StepOnePlus
Max booking time, day:
hours
Max booking time, night:
hours
No. of future bookings:

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